Fungal Isolation and Identification

Fungi are a large group of microorganisms and many cellular organisms. Fungi are divided into two major groups: unicellular fungi, which are yeasts, and multicellular fungi, which are molds or filamentous fungi. Isolation and identification of fungi is the isolation and culture of fungi and then, according to the characteristics of the colony and microscopic morphology, structure to determine the species. If necessary, through biochemical reactions, identification tests, animal inoculation, and other methods to clarify the strain. There are four types of genes encoding ribosomal RNA in the fungal genome, namely 28S rDNA, 5S rDNA, 18S rDNA, and 5.8S rDNA. 28S rDNA on the large subunit of fungi can be divided into several regions, such as D1 and D2, of which the D1/D2 region is about 500 bp in length and can be used for the identification of fungi. The fungal internal transcribed space (ITS) genes, including ITS1 and ITS2, are fast evolving, polymorphic, and suitable for identifying closely related strains.

Lifeasible can provide various tools to help customers identify fungal species, including morphological observation, physiological and biochemical identification, and molecular biology identification.

• Isolation and identification of endophytic fungi. The identification of endophytic fungi uses a combination of morphological identification and molecular biology identification. The colonies formed by different microorganisms growing on specific media generally have stable characteristics, which can be an essential basis for the classification and identification of the microorganism.
• Isolation and identification of mycorrhizal fungi. Mycorrhiza is a reciprocal symbiosis established by plant roots and fungi, which are further divided into ectomycorrhizae and endomycorrhizal based on morphological and anatomical characteristics. We provide services for the isolation and identification of mycorrhizal fungi.
• Isolation and identification of plant pathogenic fungi. Many fungi are pathogenic to wild and cultivated plants and can cause plant diseases, and fungal diseases are the largest category of plant diseases. We can provide services for isolating and identifying plant pathogenic fungi based on morphology and molecular biology.

Notes

• Test tube bacterium, glycerol bacterium, bacterium solution with volume greater than 500 µl.
• Possible taxonomic status of the samples sent known growth traits, isolation environmental conditions, purity of the DNA provided, etc.
• Whether the fungal sample is pathogenic and potentially dangerous, if your sample is somewhat pathogenic or potentially dangerous, please provide your genomic DNA.

Lifeasible can help customers isolate and identify fungi based on morphology, molecular biology, and other techniques. As your trusted partner, we can meet your needs for mycorrhizal fungi identification and isolation services and provide efficient and high-quality services. If you want to know the details, please contact us.