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Gene Editing in Diptera

Gene Editing in Diptera

Diptera is the fourth largest order of insects, 85,000 known species with a global distribution, complex habits, highly adaptable, terrestrial or aquatic, generally diurnal, with a few species active at dusk or at night. Some of these species are vectors of disease to humans or other plants and animals. Larvae have a wide and varied diet, broadly divided into phytophagous, carrion or dung feeding, predatory and parasitic. Adults feed on nectar, sap and other humus, while some groups are predatory, feeding on insects or other small animals, and some groups are predatory in both larvae and adults. There are several ways to control insects in Diptera, but gene editing technology is the most effective.

Our Services for Gene Editing in Diptera

The adults of dacus dorsalis lay eggs in the host fruit, and the larvae eat the pulp in the fruit and develop and grow, which may cause fruit drop or make the fruit lose its economic value. Anopheles sinensis is an important vector of malaria and other insect diseases in rice-growing areas. Aedes aegypti is a small to medium-sized black mosquito species that is infected through the bite and is the main biological vector of some diseases. Lifeasible provides gene editing services for Diptera including dacus dorsalis, anopheles sinensis, and aedes aegypti with zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALEN) and CRISPR/Cas9 to control these organisms.

Editable genes Relevant traits exhibited after editing
Dacus dorsalis
  • Edit reproduction-related genes to affect the profess of reproduction of larvae.
  • Edit embryo development-related genes to control embryo development in dacus dorsalis.
  • Edit the resistance-related genes to affect the resistance of dacus dorsalis to drug.
  • Edit the growth and development-related genes to affect the growth and development process of dacus dorsalis.
Anopheles sinensis
  • Edit the resistance-related genes to affect the resistant process of anopheles sinensis.
  • Edit odor perception-related genes to interfere the host-seeking process of anopheles sinensis.
  • Edit sensory-related genes to interfere the perception profess of anopheles sinensis.
  • Edit development-related genes to affect the growth and development process of anopheles sinensis.
Aedes aegypti
  • Edit immune-related genes to affect resistant ability and the innate immune system of aedes aegypti.
  • Edit genes for egg formation and egg development processes to regulate egg formation and development in aedes aegypti.
  • Edit biosynthetic pathway genes to disturb the biosynthesis and metabolism of aedes aegypti.
  • Edit reproduction-related genes to affect the reproduction behavior of aedes aegypti.
Hermetia illucens
  • Edit stunting-related genes to increase body size and improve utilization.
  • Edit wing development-related genes to reduce the difficulty of feeding and recovery.
  • Edit eye color-related genes to help new strain development.
Drosophila melanogaster
  • Transposase randomized insertion. DNA fragments can be inserted randomly in the genome to obtain transgenic Drosophila melanogaster lines with different insertion locations.
  • Integrase fixed-point insertion. Exogenous sequences can be inserted into specific attP sites in the Drosophila genome to obtain phenotypically stable transgenic lines.
  • Introduction of exogenous genes into Drosophila melanogaster for expression.
  • CRISPR-based gene editing in Drosophila melanogaster.

Our Methods for Gene Editing in Diptera

We use ZFNs, TALEN and CRISPR/Cas9 to edit some genes in dacus dorsalis, anopheles sinensis, and aedes aegypti like resistant-related genes, immune-related genes, reproduction-related genes, and growth-related genes to control these organisms.

Advantages

We use three gene editing methods to affect the profess of reproduction of larvae, the resistance to pesticides, the immune system and other growth and development of some organisms to control these pests with low risk and high efficiency.

Lifeasible provides gene editing services for Diptera including dacus dorsalis, anopheles sinensis, and aedes aegypti with ZFNs, TALEN and CRISPR/Cas9 to control these organisms. If you are interested in our services or if you have any questions, please click online inquiry for more detailed information.

Our Services are for Research Use Only. Not for Clinical Use!
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