Iris tectorum Transformation

Iris tectorum is a perennial herb native to China and introduced in Italy, Japan, and Korea. Iris tectorum is widely loved for its unique form and color. It has a long history not only in horticulture but also plays a vital role in scientific research. Iris tectorum can be used as fresh-cut and potted flowers, and its rhizomes can be used as medicine, to make essential oils and liquors, etc. It has a wide range of applications. Iris tectorum can be used as fresh cut and potted flowers, and its rhizomes can be used as medicine, essential oils, wine, etc., which has a broad application prospect. Genetic transformation of Iris tectorum using molecular biology techniques has become an essential means of studying its biological characteristics and improving varieties. We are committed to providing advanced plant genetic transformation technology, fully developing the application value of Iris tectorum, and meeting the demand for more high-quality Iris tectorum.

What do we offer?

• Selection and construction of target genes. According to the research objectives, gene sequences with specific functions are selected or designed, and expression vectors containing these genes are constructed.
• Preparation of transformation vectors. Insert the target genes into suitable transformation vectors, which usually contain elements such as promoters, terminators, and selection markers.
• Genetic transformation. The constructed vectors are introduced into Iris tectorum cells using Agrobacterium-mediated method.
• Screening and regeneration. Successfully transformed cells are screened by antibiotic screening or molecular markers and induced to regenerate to form complete plants.
• Molecular assay. The integration of target genes in the transformed plants was verified using PCR, Southern blot, and other methods.
• Phenotypic analysis. Phenotypic observation of the transformed plants to assess the effect of target gene expression and genetic stability.

Iris tectorum transformation technology platform

• Agrobacterium-mediated method. The T-DNA region of Agrobacterium rhizogenes was utilized to introduce target genes into Iris tectorum cells, and this method has shown a high transformation rate and more applications in the genetic transformation of Iris tectorum.
• Gene gun method. The physical transformation of genes is achieved by shooting microparticles containing target genes into Iris tectorum cells using high-pressure gas. The gene gun method in Iris tectorum genetic transformation has technical advantages such as relatively simple operation, no need for complex tissue culture, and the ability to obtain transgenic plants quickly.

Our research objectives for Iris tectorum transformation

• Improvement of ornamental value. Traits such as color, morphology, and flowering period of Iris tectorum can be improved through genetic transformation to meet the demand of landscaping and flower markets.
• Enhancement of stress resistance. To study the physiological response mechanism of Iris tectorum under drought, high temperature, and other adversities and to improve its ability to resist drought, heat, and other adversities through genetic transformation.
• Breeding research. By combining genetic transformation technology and traditional breeding methods, the breeding process of new Iris tectorum varieties can be accelerated and the breeding efficiency can be improved.
• Molecular mechanism research. Using genetic transformation technology, we can study the molecular mechanism of Iris tectorum, such as regulating the expression of heat stress factor genes, and the role of Ca2+ signaling in plant heat tolerance.

Why choose us?

• Specialized technical team. Our experts and technical team all have rich research and service experience in plant genetic transformation research.
• Advanced laboratory equipment. We have advanced experimental equipment to ensure the success rate of transformation.
• Customized technical services. We can provide customized solutions for plant genetic transformation according to customer project requirements.
• Strict quality control. We have established a systematic transformation system for each plant and carry out strict quality control for each transformation step to ensure the efficiency and accuracy of transformation.

Our service workflow

Lifeasible has integrated gene editing and genetic transformation technologies and established advanced experimental technology platforms such as molecular genetic transformation and plant tissue culture. We have also developed and established a multi-species high-throughput gene editing and genetic transformation platform, which can significantly meet the needs of researchers in plant genetic transformation. If you are interested in us, please feel free to contact us.