Mechanism Analysis of Host Cell Wall Degradation by Effector Proteins

Plant cell wall is the natural physical barrier of plant cells, which plays the role of protecting plant cells. Its main components include cellulose, hemicellulose, and a pectin layer. In the process of infection, nematodes destroy plant tissues through mechanical puncture of oral needles and secrete a series of cell wall modifying proteins, which are transported to host cells to degrade plant cell walls, helping nematodes break through the first physical barrier of plants.

Lifeasible has enriched plant science experience, performing various strategies to help our clients analyze the mechanism of host cell wall degradation by effector proteins. Our scientists and expert technicians have been dedicated to customizing your program to meet your requirements.

Degradation of Host Cell Wall by Hydrolase

• Interwoven networks of cellulose and pectin are the main components of plant cell walls, making them recalcitrant structures that can only be degraded by organisms producing a mix of synergistically acting enzymes.
• Lifeasible provides degradation analysis of host cell wall by hydrolase, for example, β-1,4-endoglucanase and β-1,3-endoglucanase that are secreted by plant nematodes to hydrolyze cellulose, pectate lyase, and polygalacturonase that enables hydrolyze cell wall pectic substance, and β-1,4-endoxylanase that can hydrolyze of hemicellulose.

Degradation of Host Cell Wall by Expansin Proteins

Fig.1 Extension activity of Gr-EXP1 on plant cell walls.

• Plant cell walls are a rigid network of interwoven polymers. Many organisms that rely on plants as a food source use a variety of proteins with or without enzyme activity to break the covalent bonds in this polysaccharide structure. Expansion proteins, which have so far been identified only in plants, rapidly induce the extension of plant cell walls by weakening the non-covalent interactions that help to maintain their integrity.
• We provide degradation analysis of the host cell wall by expansion proteins using whole-mount in situ hybridization, such as Gr-Exp1 which define the expansion superfamily as the first non-plant gene.

Degradation of Host Cell Wall by Cellulose Binding Proteins

• Cellulose-binding proteins have been reported to promote cell wall degradation by relaxing cell walls and breaking non-covalent bonds between polysaccharide chains.
• We provide degradation of the host cell wall by cellulose-binding proteins, which interact directly with pectin methylesterase 3 (PEM3), reducing the pectin methyl ester exchange reaction and accelerating the activity of other cell wall degrading enzymes.

Lifeasible is pleased to share our cutting-edge technology and extensive expertise in plant science to facilitate our clients' research and project development. We can offer high-quality customized services by adjusting protocols to meet specific requirements. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.