Mechanism Analysis of Nematodes Overcoming Host Physical Barrier

The host cell wall is the main obstacle to the invasion and feeding of pine wood nematode. During nematode invasion, host lignin and other secondary cell wall-related substances synthetase genes are up-regulated, which induces host cell wall thickening and restricts the invasion of nematode. Thus, to successfully invade, nematodes secrete cell wall degrading enzymes that destroy plant cell walls and overcome host physical barriers. Cell wall degradation enzymes secreted by nematodes mainly include cellulase, glycosyl hydrolase, pectate lyases, expansin, α-L-glycosidase, etc.

Lifeasible offers comprehensive services covering a wide range of cutting-edge technologies to advance your projects. Our scientists have developed a series of innovative solutions to help analyze the mechanisms by which nematodes overcome physical host barriers.

Nematodes Overcoming Host Physical Barrier by Cellulase

• Cellulase has the activity of cellulose and hemi-cellulase to degrade the host cell walls, so it is considered the key enzyme in the degradation of the plant cell wall by nematodes.

Fig.1 Barrier to parasitic nematodes.

• Lifeasible provides mechanism analysis of nematodes overcoming host physical barrier by cellulase, including the site of cellulase secretion in nematode and the site of cell wall degradation in plants. We also help to investigate the role of cellulase in the fungus-feeding phase of saprophytic late infection.
• In addition, we provide RNA interference techniques to explore the effects of cellulase genes on migration rates and pathogenicity of nematodes.

Nematodes Overcoming Host Physical Barrier by Glycosyl Hydrolase

• Glycosyl hydrolase is one of the main cell wall degrading enzymes in plant nematodes. Nematodes possess multiple glycosyl hydrolase genes, which may have been acquired from fungi by horizontal gene transfer.
• We assist in the analysis of copy and tandem repeats in the nematode genome, where copy number is associated with the pathogenicity of nematodes, and differences in tandem repeats can lead to changes in protein sequences that also affect pathogenicity.

Nematodes Overcoming Host Physical Barrier by Pectate Lyases

• Pectin lyase also plays an important role in the pathogenesis of nematodes. The pectin lyase gene (Bx-pell), which has poly galacturonic acid activity, is highly expressed in the pathogenesis of nematodes.
• We help to study the specific expression of this gene, in addition the effect of this gene on the reproductive rate, mobility, and pathogenicity of nematodes by RNAi silencing technology.

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