Metabolic flux analysis uses stable isotope labeling of specific molecules and traces their metabolic processes in organisms to obtain dynamic information about metabolites in metabolic pathways. Metabolic flux analysis can comprehensively explain the process of substance metabolism and plays a vital role in the identification of metabolic pathways, metabolic mechanism analysis and metabolic engineering.

Lifeasible focuses on agricultural bioscience research; we provide advanced and reliable metabolic flux analysis service, relying on a multi-dimensional technology platform and interdisciplinary approach to provide precise support for agricultural biologics research and development, microbial metabolic engineering, crop metabolism regulation, and other fields.

Explore Our Metabolic Flux Analysis

Analysis of common metabolic pathways

• Glycolysis pathway analysis. By tracking and analyzing the changes in the metabolic flow of glucose during glycolysis, we can help researchers understand the energy metabolism status of crops under different environmental conditions and provide a basis for optimizing planting conditions.
• Tricarboxylic acid cycle analysis. Through the detection of citric acid, α-ketoglutarate, and other metabolites, we can analyze their metabolic fluxes in the tricarboxylic acid cycle, evaluate the respiration intensity and energy production efficiency of crops, and help study the growth and development of crops and the response mechanism to adversity.
• Pentose phosphate pathway analysis. By analyzing metabolites such as 6-phosphogluconic acid, we can help researchers understand the metabolic fluxes in the synthesis of ribose, NADPH, and other important biomolecules in crops, which provides data support for the study of crop growth metabolism and antioxidant mechanisms.

Crop quality related analysis

• Amino acid metabolism analysis. Detecting amino acids such as aspartic acid and glutamine, analyzing their metabolic flow in protein synthesis, nitrogen metabolism, and other processes to provide a basis for cultivating crop varieties with high protein content, which helps improve crops' nutritional value.
• Fatty acid metabolism analysis. Analyze a variety of fatty acids to understand the metabolic flow of crops in the process of oil synthesis, energy storage, etc., to provide technical support for improving the oil quality of crops and increasing the oil yield of oil crops.

Plant adversity response analysis

• Metabolic flux analysis under adverse conditions. Metabolic flux analysis in drought, salinity, and other adverse conditions analyze the changes of metabolic flow in the crop, reveal the crop's resistance mechanism, and provides a theoretical basis for cultivating crop varieties with strong resistance to adversity.
• Antioxidant metabolic flux analysis. Analyze the role of superoxide dismutase, catalase, and other antioxidant enzymes in the metabolic flow to understand the antioxidant defense mechanism of crops under adverse conditions and to provide ideas for improving crop resistance.

Crop growth regulation analysis

• Metabolic flux analysis of plant hormones. Analyzing the changes of growth hormone, gibberellin, and other plant hormones in the metabolic flow reveals their regulatory mechanisms in the process of crop growth and development, providing theoretical support for the precise regulation of crop growth.
• Metabolic flux analysis of signal transduction. We study the changes of metabolic flow in the signal transduction pathway of crops under external stimuli, which can be used to understand the perception and response mechanism of crops to environmental changes and provide a basis for optimizing planting management measures.

Our Service Process of Metabolic Flux Analysis

• Labeling experiments. We optimize experimental conditions and introduce stable isotope-labeled substrates.
• Sample processing. We extract metabolites and remove interfering substances through rapid quenching of metabolic reactions.
• Mass spectrometry. Based on LC-MS/MS or GC-MS platforms, we accurately analyze metabolite isotope distribution.
• Flux calculation. Combined with metabolic network models, we use mathematical models to derive metabolic fluxes.

Fig.2 Process of our service. (Lifeasible)

Highlights of Our Metabolic Flux Analysis

• High precision. We use multi-substrate labeling combinations with parallel experiment design to reduce background noise.
• Multi-omics integration. We support metabolic flux analysis with transcriptome and proteome to reveal multi-dimensional regulatory mechanisms.
• Interdisciplinary experience. Our expert technical team has specialized knowledge and rich experience in metabolic flux analysis, which can help researchers to analyze complex metabolic networks globally.

Lifeasible provides customers with reliable and efficient metabolic flux analysis services and helps with the translation of scientific research results and industrial upgrading. If you are interested, please feel free to contact us.