Microalgae Expression System

Microalgae, also known as microphytes, are a multilineage group ranging from prokaryotes to eukaryotes. As eukaryotes, microalgae can undergo post-translational modifications such as glycosylation, which is often necessary for protein function. In addition, microalgae are free from toxins and animal source-induced problems in recombinant protein production, making microalgal expression systems more biologically safe. With post-translational modifications and reduced contamination by disease-causing compounds, microalgae expression systems have shown great potential for application in life science research and have gained much attention from researchers.

Lifeasible has many years of service in plant protein expression system research, especially in microalgae expression systems, partner with us and let's push the research progress of the microalgae expression system together.

Advantages of Microalgae Expression System

• Short production time. Microalgae have microbe-like characteristics of rapid growth, making them very practical in gene expression systems for rapidly producing desired proteins or metabolites.
• Cost-effective expression system. Microalgae can express target proteins quickly, and with simple cultivation, the products expressed by microalgae are easier to purify.
• Easy to handle and cultivate. Microalgae have a relatively simple culture medium, usually requiring only inorganic salts and light, which simplifies the cultivation process and reduces costs.
• Biosafe. Protein products produced by microalgae expression systems are typically free of endotoxins and exotoxins, increasing product safety.

Our Service for Microalgae Expression System

We provide a one-stop microalgae recombinant protein production service covering the whole process from gene cloning to protein expression and purification in the microalgae system, which provides excellent convenience for researchers.

• Codon bias in the nuclear genome and organelles is an essential factor that affects the efficiency of protein expression. In eukaryotes, codon usage is not completely random, but there is a degree of bias. This bias may affect mRNA stability and translation efficiency. This requires us to repeatedly consider the codon bias of the host cell and select the most suitable codon to improve the expression level of the target protein when designing gene clones.
• Selecting promoters and regulatory elements controlling transgenes is crucial for protein expression. Promoters are the starting point for controlling gene expression, while regulatory elements such as untranslated regions (UTRs) can further regulate gene expression. We optimize the selection of promoters and regulatory elements based on the properties of the target protein and the characteristics of the host cell. For example, we will optimize the chloroplast promoter and the untranslated region (UTR) by fusing the 16s ribosomal promoter to the atpA 5'UTR or fusing the hsp70 promoter to the rbcs2 promoter; the efficiency of protein expression can be further improved.
• The selection of markers is an important tool used to screen successfully transformed cells. We offer several enhanced fluorescent proteins that can allow researchers to easily identify and screen cells that successfully express target proteins by fluorescence microscopy or flow cytometry. This selective labeling can effectively improve screening efficiency and simplify subsequent protein purification and functional studies.

Why Choose Us?

• Professional technical team. We have experts in transgenic microalgae and protein expression purification who have profound professional knowledge and rich practical experience and can provide professional technical support and services for our customers.
• One-stop service. We provide a one-stop service from gene cloning to protein expression purification in microalgae systems, to save a lot of time and energy for our customers and improve the efficiency of research and production.
• Advanced molecular tools. We utilize advanced molecular tools, such as selecting powerful genetic elements and optimizing protein production strategies to improve the expression efficiency and yield of recombinant proteins.
• High-throughput technologies. We have the high-throughput technologies needed to select the best expression constructs and transformants to rapidly screen for optimal expression systems and accelerate the development process.

Lifeasible has accumulated rich service experience in microalgae expression system service, and our professional technical team can provide high-quality microalgae expression systems and many related special services. If you are interested, please feel free to contact us.