Microalgal Proteomics

The advent of the omics era has revolutionized the study of microalgal biology. Among them, high-throughput proteomics complement microalgal omics and provides accurate functional insights into the accompanying static and dynamic physiological environments. Here, Lifeasible provides professional solutions for microalgal proteomics.

Microalgal Genomics

Eukaryotic microalgae are a rich source of commercially important metabolites, including lipids, pigments, sugars, amino acids, and enzymes. However, their inherent genetic potential is often insufficient to support high production of target metabolites. Rapid advances in high-throughput sequencing and spectroscopy technologies have greatly facilitated the identification of new targets for microalgal strain engineering. Proteomics has been established as a powerful tool to explore the mechanisms involved in many biological processes and network functions by providing information about proteins, including post-translational modifications, subcellular localization, and protein-protein interactions. The first proteomic analysis in microalgae was performed in the unicellular freshwater green algae Chlamydomonas reinhardtii, which was selected as a model organism. An integrated approach of proteomics, genomics, and bioinformatics provides sustainable solutions for microalgae.

Fig 1. High-throughput proteomics and metabolomic studies guide the re-engineering of metabolic pathways in eukaryotic microalgae. (Chakdar H, et al., 2021)

Customized Solutions

Genomics and transcriptomics alone are insufficient to understand microalgae's complex biology and should be complemented with proteomic approaches. Our scientists have successfully established model organisms such as the unicellular freshwater green algae Chlamydomonas reinhardtii, diatom P. tricornutum, and on oleaginous microalgae to perform microalgal proteomic analysis. Lifeasible offers customized microalgal proteomics solutions to analyze post-translational modifications, and identify proteins at different levels in sub-polyprotein complexes and their location in different cell types and tissues. We offer microalgal proteomics, including but not limited to the following:

• Isolation of Microalgal Proteins
• Analysis of Post-Translational Modifications of Microalgal Proteins
• Analysis of Microalgal Proteins Interactions
• Subcellular Localization of Microalgal Proteins

The Unique Advantages of Our Microalgal Proteomics

• Based on our advanced birdshot proteomics technology, we can help you isolate and compare complex protein mixtures of microalgae and identify some unknown genes and proteins.
• Allows evaluation of the proteomic profile of microalgal regulation in response to different growth conditions and the establishment of comparative transcriptomic profiles against genes encoding structural proteins.

Our Available Techniques

• Efficient, time-saving, and labor-saving bird shot proteomics technique.
• Blue natural polyacrylamide gel electrophoresis (BN-PAGE).
• Two-dimensional electrophoresis (2D SDS-PAGE ).
• 2D gel electrophoresis combined with immunoblotting (2D-IS).
• Mass spectrometry.
• GeLC/MS/MS method.
• MRM (Multiple Reaction Monitoring) and SRM (Selective Reaction Monitoring) quantification methods.
• MALDI-TOF-MS method.
• Stable isotope labeling of amino acids (SILAC).

At Lifeasible, our proteomics services for microalgae allow us to isolate and identify proteins from complex protein mixtures in a reliable manner, providing promising feedstocks for the on-demand production of renewable fuels and chemical intermediates. We are your trusted partner in all aspects of microalgae research. If you are interested in our solutions for microalgae proteomics, please do not hesitate to contact us.