Mollusks Protein Purification Services

Protein purification technology is an essential technique in the field of biological research. To study a particular protein, it is first necessary to isolate and purify it from mollusks. Protein purification methods mainly use the similarities and differences between different proteins. Based on the similarity between the proteins, the non-protein material can be removed, and then the target protein can be isolated based on the differences between the proteins.

Protein Purification Methods

Our purification strategy focuses on improving protein purity while reducing the loss of protein expression. Therefore, we often use a mixture of purification methods commonly used in the following table.

Our Services

Lifeasible can provide protein purification services for mollusks to meet the different needs of our clients, such as the need to purify proteins for structural analysis and the need to purify proteins obtained from mollusks, as follows.

• Soluble protein expression purification

Protein sequence provided by the customer, Lifeasible optimization and gene synthesis, and expression purification to obtain soluble proteins.

• Label-free protein services

Comprehensive analysis of protein properties, combined with molecular sieves, ion columns, hydrophobic chromatography, and comprehensive development of purification plans.

• Sample preparation and purification steps

To obtain proteins of sufficient purity and quantity efficiently and economically, it is important to set the framework and working time frame for the purity, quantity, and maintenance of biological activity and limited economy of the purified material. The development of the sample preparation and extraction process should take precedence over the first step of purification using chromatographic techniques. A three-step purification strategy can be chosen for the analytical sample and sample preparation step when background information on the sample is available.

The three stages of purification of samples include sample capture, medium purification, and fine purification of samples.

In the three-step purification strategy, specific targets are assigned at each step of the purification process.

• In the target capture stage, the sample is separated, concentrated, and stabilized for the target.
• In the medium purification stage, many impurities are removed from the sample, such as other proteins and nucleic acids, endotoxins, and viruses.
• In the fine purification stage, a higher purity is achieved by removing any residual trace impurities or closely related substances.

If you are interested in our services, or if the service you want is not listed, please feel free to contact us, and we will get back to you as soon as possible.