Protein purification technology is an essential technique in the field of biological research. To study a particular protein, it is first necessary to isolate and purify it from mollusks. Protein purification methods mainly use the similarities and differences between different proteins. Based on the similarity between the proteins, the non-protein material can be removed, and then the target protein can be isolated based on the differences between the proteins.
Our purification strategy focuses on improving protein purity while reducing the loss of protein expression. Therefore, we often use a mixture of purification methods commonly used in the following table.
| Purification Methods | Brief description |
| Affinity purification | Separation of proteins using the affinity between the target protein and its specific ligand. |
| Ion exchange chromatography | Charge binding of an ion exchanger to the target protein and elution of the target protein by changing the pH or increasing the ionic strength of the buffer. |
| Gel filtration chromatography | Separation is mainly based on protein molecular weight and molecular shape. |
| Hydrophobic chromatography | Separation and purification using the reversible interaction of protein bound to hydrophobic ligands. |
| High-performance liquid chromatography | Compared with conventional liquid chromatography columns, it has the advantages of high separation efficiency, high detection sensitivity, and fast analysis speed. |
Lifeasible can provide protein purification services for mollusks to meet the different needs of our clients, such as the need to purify proteins for structural analysis and the need to purify proteins obtained from mollusks, as follows.
Protein sequence provided by the customer, Lifeasible optimization and gene synthesis, and expression purification to obtain soluble proteins.
Comprehensive analysis of protein properties, combined with molecular sieves, ion columns, hydrophobic chromatography, and comprehensive development of purification plans.
To obtain proteins of sufficient purity and quantity efficiently and economically, it is important to set the framework and working time frame for the purity, quantity, and maintenance of biological activity and limited economy of the purified material. The development of the sample preparation and extraction process should take precedence over the first step of purification using chromatographic techniques. A three-step purification strategy can be chosen for the analytical sample and sample preparation step when background information on the sample is available.
The three stages of purification of samples include sample capture, medium purification, and fine purification of samples.
In the three-step purification strategy, specific targets are assigned at each step of the purification process.
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Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
