Nanotechnology for Plant Pathogen Detection

Nanotechnology allows the study of the properties and applications of samples with structural dimensions in the range of 1-100 nm. Nanoparticles have wonderful applications in susceptible biomolecular detection, disease diagnosis, and antimicrobial and therapeutic compounds. Pathogens can infect plants through various pathways, and when plants cannot fight them off, they will become infested with pathogens. Lifeasible's aggressive introduction of nanotechnology in plant-pathogen detection is an extremely promising means of targeting integrated plant disease management.

We use an ultra-highly sensitive flow-through assay for quantitative multi-parameter characterization at the single particle level, which allows rapid characterization of biochemical traits in plant pathogens. Our simultaneous detection of individual nanoparticle scattering and multicolor fluorescence enables rapid multi-parameter simultaneous quantitative characterization of traits such as particle size and its distribution, particle concentration, the number of surface-coupled targeting probe molecules and their distribution in nanobiological samples, and correlation analysis between parameters. We provide solutions for quantitative and qualitative analysis of natural biological nanoparticles, including extracellular vesicles, viruses, bacteria, and subcellular structures, which can fully meet your needs for plant pathogen detection.

Characterization of viruses in plants

Plant viruses are a devastating class of plant pathogens for which researchers lack effective management options due to their genetic diversity, rapid reproduction, and evolution. We offer nano-flow detection solutions to characterize the species, particle size, and internal composition of plant viruses or virus-like particles with the advantage of high resolution and high throughput.

• We can achieve size discrimination of different types of virus particles, high-throughput detection of different components of viruses, and dynamic monitoring of viral DNA release by measuring the intensity of scattered light from individual virus particles.
• With the help of nucleic acid staining and dual-parameter detection of scattering and fluorescence, we can achieve rapid identification of intact viral particles, viral capsids, and free viral nucleic acids at the single-particle level, which will provide a suitable means of characterization for the development of nanomedicines and vaccines for applications in plant disease control.

Detection of bacteria in plants

In plants, bacteria cause leaf spots, fire blight, and wilt. However, bacteria have small particle sizes, simple internal structures, and a low number of self-expressed biomolecules, which cannot label a sufficient number of fluorescent probes. We provide a nano-flow assay solution that can overcome the significant limitations of traditional flow cytometry in the highly sensitive detection of bacteria. It has a low fluorescence detection limit and can accurately detect the weak fluorescent signals produced by bacteria.

Lifeasible's use of nanotechnology offers a promising avenue for the sustainable protection of plants against phytopathogens. Not only can nanoflow solutions be used to detect plant diseases, but we are also developing nano-formulations to reduce pesticide use and metal nano-formulations to control plant diseases to increase crop yields and restore and improve soil quality. Please feel free to contact us for information on how nanotechnology can be applied to plant pathogen detection.