Organic acids (OA) are a class of highly acidic organic substances that exist in nature as salts, esters, or free forms. The quantitative analysis of organic acids is essential for ensuring the quality of fruit and vegetable products and studying biological metabolic pathways. Lifeasible provides comprehensive targeted metabolism services for agricultural research, especially in the quantitative analysis of organic acids, and this assay plays an essential role in helping researchers accurately quantify organic acids in fruit and vegetable tissues, microbial fermentation broths, and soils.

Importance of Organic Acids Quantification

• The quantification of organic acid content in fruits and vegetables plays an important role in the study of fruit quality and edible taste.
• Organic acids in the root system of fruits and vegetables can be directly involved in respiration, photosynthesis and metabolism of organic compounds such as aromatic hydrocarbons, and quantification of organic acids in fruits and vegetables can determine the optimal preservation conditions.
• Organic acids are important products of microbial fermentation, and quantitative analysis of organic acids can be used to understand the metabolic pathways of strains and further determine the significance of metabolic network pathways within the bacterium.
• Organic acid quantification services can be used to study the treatment of heavy metal pollution in soil and soil remediation.

Our Service for Organic Acids Quantification

Quantification of organic acids in fruits and vegetables

High-performance liquid chromatography (HPLC) is a commonly used method for quantification of organic acids in fruits and vegetables. This method takes advantage of the fact that organic acids are readily soluble in water, alcohols, and dyes. It extracts the organic acids through these solvents, then separates them using a chromatographic column and analyzes them quantitatively by a UV detector. The quantification of organic acids performed by HPLC release is characterized by high sensitivity and separation, and it is suitable for determining organic acids in a wide range of fruits, vegetables, and their products.

Quantification of organic acids in bacterial strains

Quantification of organic acids in strains can be achieved by monitoring changes in pH during fermentation. This method utilizes the characteristics of pH changes during organic acid production and combines pH indicators and high-throughput techniques to establish a screening method. We carried out initial screening of the strains to be screened in the medium containing specific pH indicators and selected the strains with larger color change circles for fermentation verification, using this method to screen strains with high organic acid production quickly.

Quantification of organic acids in soil

The quantification of organic acids in soil usually adopts the acid-base titration method, the principle of which is to use an acid-base neutralization reaction to determine the content of organic acids. The main sources of soil organic acids include plant residues, root excretions, and microbial metabolites, which can provide energy and nutrients to the soil. Commonly used instruments for organic acid determination include UV-Vis spectrophotometer and pH meter, etc. The organic acid content is calculated by measuring the spectral absorption value and pH value of the samples.

In addition, we also use chromatography-mass spectrometry to quantify organic acids, which is used to more accurately identify and analyze the components of different types of organic acids.

Fig.2 Our service process. (Lifeasible)

Sample Request

• Type. Plant tissue (≥1.0 g/sample recommended, minimum not less than 0.5 g), liquid (≥10 ml/sample).
• Preservation. Liquid nitrogen quick-freeze and store at -80°C.
• Transportation. Dry ice transportation.

Application of Organic Acids Quantification

• Plant adversity physiology research
• Plant biotic and abiotic stress research
• Photomorphogenesis
• Plant growth and development
• Metabolic regulation pathway analysis
• Microbial metabolite research
• Food standards testing

Highlights of Our Organic Acids Quantification

• Real-time project tracking and management. We can track the progress of a project in real-time, ensuring that our clients are aware of every stage of the project.
• Efficient issue resolution. Our project management team is committed to responding to and handling client feedback efficiently.
• Advanced analytics. We employ various advanced technologies, especially based on our GC-MS analytical platform, which enables us to perform simultaneous qualitative and quantitative analyses of a wide range of organic acids.
• Strict quality control. We implement strict quality control measures for each project to ensure adequate evaluation before analysis.

Lifeasible is committed to providing high-quality quantitative organic acid analysis services to help our clients' biological projects. If you are interested, please feel free to contact us.