Plant systems and evolutionary biology is an increasingly comprehensive field of study that investigates the origins of plant species diversity, the mechanisms of diversification, and their evolution. Plants have evolved through a very complex evolutionary process, during which many genes were generated along potentially divergent routes. Phylogenetic network diagrams provide a clear picture of the complex web of evolutionary relationships, including both vertical and horizontal evolution.
Lifeasible, a leading biotechnology company, has been working intensively on the evolutionary analysis of plants. Our advanced plant gene sequencing platform and diverse bioinformatics tools enable us to reveal the origin and evolutionary history of new plant traits based on comparative evolutionary genomics. We provide our clients with phylogenetic and molecular evolutionary analysis services based on a "scientific, precise, and customizable" service philosophy.
During the evolution of a species, there are two main types of evolutionary routes for different genes: vertical evolutionary processes and horizontal evolutionary processes. Our analysis service can summarize these two evolutionary processes in a network diagram, helping our clients to gain a clearer understanding of the molecular evolution of plants.
The target plant samples were quickly put into liquid nitrogen and frozen, and total RNA was extracted. The cDNA was amplified using PCR and sequenced after constructing a sequencing library.
After high-throughput sequencing, the raw image data was converted into sequence data, i.e., raw reads, and stored in FASTQ format. The clean data was obtained by removing splice sequences and low-quality sequences at both ends, and the open reading frame (ORF) prediction was performed to obtain the best ORF region in the sequence.
It is essential to assess whether the transcript sequences obtained from sequencing assemblies cover all possible transcripts. This is directly related to the size and completeness of the data in the phylogenetic analysis and, thus, to the phylogenetic tree's topology.
Phylogenetic analyses were performed using tandem and concatenation analyses.
With the rapid development of molecular biology, molecular data have become the main trait for phylogenetic analysis. However, as research progresses, phylogenetic trees from phylogenetic analyses of the same taxon using different gene sequences vary from one researcher to another. Our analysis service enables the use of phylogenetic networks to accurately reconstruct gene network relationships during the evolution of species.
Lifeasible provides a one-stop shop for plant phylogenetic and molecular evolutionary analysis, from sequencing to bioinformatics. Please contact us directly if you require further information.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
