Plant Subcellular Organelles Cryo-Electron Microscopy Imaging

Plant Subcellular Organelles Cryo-Electron Microscopy Imaging

Fluorescent microscopy is a cornerstone of modern cell biology. However, fluorescent labeling can result in the loss of the surrounding cellular environment, and certain fluorescent stains may affect the natural structure of cells or organelles. Therefore, when you want to understand the ultrastructure inside tissues and cells, cryo-electron microscopic (Cryo-EM) imaging is the way to go. Cryo-EM is an advanced imaging technique widely used to explore the structure of subcellular organelles.

Plant subcellular organelle cryo-electron microscopy imaging.

As a professional bioimaging company, Lifeasible has built up a cutting-edge cryo-electron microscopy imaging technology platform. We provide one-stop cryo-electron microscopic imaging services for botanists worldwide to help them elucidate the 3D structure of subcellular organelles and protein complexes, as well as organelle interactions.

Why Choose Cryo-Electron Microscopy (Cryo-EM)?

Cryo-EM can help us to observe the three-dimensional structure of plant subcellular organelles in different states, as well as all complex conformations, structures, and modified forms of proteins in subcellular organelles. At the same time, Cryo-EM only requires a small sample and does not require crystallization of biological samples, avoiding the impact of purity and heterogeneity issues on research.

What We Offer

Depending on your needs, our Cryo-EM technology platform can provide you with several imaging services, such as cryo-transmission electron microscopy, cryo-scanning electron microscopy, cryo-electron tomography, and cryo-etching electron microscopy.

  • Cryo-Transmission Electron Microscopy (Cryo-TEM)

Cryo-TEM usually adds a sample freezing device to an ordinary transmission electron microscope to observe temperature-sensitive samples such as proteins and biological sections. By freezing the sample, the damage to the sample by the electron beam can be reduced, and the deformation of the sample can be reduced, thereby obtaining a more realistic sample morphology.

  • Cryo-Scanning Electron Microscopy (Cryo-SEM)

Cryo-SEM technique is a fast, reliable, and effective method to overcome the problem of sample water content. Cryo-SEM is the most effective way to prevent moisture loss from the sample, and it can be used in any vacuum.

  • Cryo-Electron Tomography (Cryo-ET)

Cryo-ET images at nanoscale resolution, producing three-dimensional (3D) images from a series of tilted Cryo-EM images. It is commonly used for many structures inside cells, such as subcellular organelles.

  • Cryo-Etching Electron Microscopy (Freeze-Etching)

Freeze-etching technology is used for microstructural studies in fields such as cell biology. The sample is frozen so that the microstructure is close to that of a living organism; after freeze-etching, the microstructure of the different cleavage surfaces can be observed, allowing the study of the membrane structure and inclusions within the cell.

Our Facilities

With the rapid development of science and technology, fluorescence microscopy imaging is becoming more and more widely used in the biomedical field. Our imaging technology platform offers the following types of fluorescence microscopes.

  • Automatic block trimmer
  • Ultra-thin sectioning machine
  • Cryo-electron microscope systems
  • Transmission electron microscopy
  • Cryo-transmission electron microscopy
  • Ion sputtering machine
  • Fully automatic staining machine
  • Automatic tissue embedding machine
  • Field emission scanning electron microscopy
  • Microwave electron microscope tissue processor

Applications

  • Plant Biology
  • Structural biology of plant subcellular organelles
  • Mitochondrial observation: altered energy metabolism
  • Chloroplast, vesicle observation: altered photosynthetic activity
  • Ribosome, rough endoplasmic reticulum observation: altered protein synthesis, etc.

Our Service Process

Service flowchart. - Lifeasible

Lifeasible is committed to providing robust technical support for observing plant subcellular organelles. If you are interested in our plant subcellular organelle cryo-electron microscopy imaging services, please feel free to contact us. We look forward to working with you.

Our products/services are For Research Use Only. Not For Clinical Use!
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