In agricultural research, in-depth investigation of the gene expression regulation mechanism of microorganisms in different environments is of key significance to enhancing crop yield, strengthening the resistance to pests and diseases, and promoting ecological balance. Prokaryotic transcriptome sequencing focuses on all transcripts of prokaryotic organisms in a specific state, including mRNA and non-coding RNA. It can accurately capture the dynamics of gene expression, which can provide an important basis for the application of agricultural microorganisms and research on the mechanism.

Lifeasible has many years of experience in the field of agricultural transcriptome research and services, based on the advanced sequencing platform, we construct strand-specific libraries, aiming to study all the mRNAs transcribed by prokaryotic organisms at a certain period or under a certain environmental condition, so far, our service has been used in the study of microbial stress response, metabolism regulation, and pathogenic mechanism research.

Explore Our Prokaryotic Transcriptome Sequencing

Experimental design and technical support

• After getting the samples, our expert team will perform quality testing and quantitative analysis of RNA to ensure that its integrity, purity, and concentration meet the sequencing requirements.
• For the characteristics of prokaryotic organisms, we use specific rRNA removal probes to efficiently remove rRNA and increase mRNA enrichment and then construct strand-specific libraries for sequencing using high-throughput sequencing platforms.

The highlights of our services are the optimization of rRNA removal protocols for different species to ensure high-quality libraries for low starting volume samples and the ability of our sequencing to meet the demands of high-resolution analysis.

Bioinformatics analysis

• Basic analysis. Data quality control, reference genome comparison, and gene expression quantification.
• Advanced analysis. Differential gene identification, functional enrichment, new transcript prediction, non-coding RNA mining, and co-expression network construction.
• Personalized service. We support transcriptome analysis for species without reference genomes.

Application of Prokaryotic Transcriptome Sequencing

• Environmental response mechanisms. To study bacteria's gene expression regulatory network under extreme temperature, pH, or nutrient stress.
• Ecological research. Analyze the functional gene expression of microbial communities in pollutant degradation or carbon and nitrogen cycling.
• Cell cycle and growth regulation studies. Sequencing of the prokaryotic transcriptome can be used to study the growth cycle, division, and cell cycle regulation in bacteria.

Our Service Workflow

• RNA extraction. Total RNA is extracted from prokaryotic samples to ensure integrity and purity.
• RNA quality testing. Professional equipment is used for RNA quality testing, including concentration, purity, and integrity analysis, to ensure that it meets the requirements for library construction.
• rRNA removal. The rRNA removal probes are used to remove rRNA and enrich mRNA efficiently.
• Library construction. Fragmentation, reverse transcription, and strand-specific library construction are performed on the removed mRNA to prepare high-quality sequencing libraries.
• Sequencing. The constructed library is loaded onto a high-throughput sequencing platform and sequenced bipartite, generating a large amount of sequencing data.
• Data analysis. We also provide data analysis services.

Fig.2 Our service process. (Lifeasible)

Highlights of Our Prokaryotic Transcriptome Sequencing

• Low sample starting volume.
• Fast cycle time, good quality, and high stability.
• Provide comprehensive, accurate, and high-quality transcriptome analysis results.
• Professional program design and strict quality control.

Lifeasible utilizes an advanced high-throughput sequencing platform capable of rapidly generating large amounts of high-quality sequencing data. Meanwhile, we are equipped with a professional bioinformatics analysis platform with powerful computational capabilities and rich analysis tools to ensure the accuracy and reliability of data analysis. If you are interested, please feel free to contact us.