Proteomic Analysis of Plants Endoplasmic Reticulum

Proteome refers to all the proteins expressed by the genome of a cell or tissue. The proteome is a dynamic concept that changes with tissues, organs, and even the environment. It varies not only in different tissues or cells of the same organism but also in different developmental stages of the same organism. Methods for proteomics research from the early two-dimensional gel electrophoresis and mass spectrometry to the present protein post-translational modification, protein chip, yeast two-hybrid, protein intracellular translocation, and so on. In recent years, it also plays a vital role in plant protein research.

Lifeasible develops an advanced platform equipped with advanced instruments and professional staff to perform proteomic analysis with a high standard. We customize featured services according to customers' needs. We deliver satisfactory and reliable results and reports on time to our customers worldwide.

Proteomic Analysis Methods of Plant Endoplasmic Reticulum

• Lifeasible offers protein quantification via two-dimensional gel electrophoresis, the most commonly used technique in proteomic analysis. The technique separates proteins in two steps, the first step is isoelectric focusing, which separates proteins according to their isoelectric points, and the second step is SDS-polyacrylamide gel electrophoresis, which separates proteins by molecular weight.
• Based on the method described above, we also obtain further sensitivity by covalently labeling the protein with a fluorescent cy dye prior to separation. This method, known as two-dimensional difference gel electrophoresis, improves the quality and quantity of protein spots and provides a more reliable gel match.
• We also offer mass spectrometry (MS) -based quantitative strategies to support relative and absolute protein quantification. TMT and iTRAQ are currently the most widely used labeling techniques because they can be used for differential quantification of post-translational modifications of different proteins.
• In addition, we offer label-free LC-MS / MS technology that allows a wide range of protein quantification with simple and short sample preparation.

Proteomic Analysis Workflows of Plant Endoplasmic Reticulum

Fig.1 Proteomic analysis of ER proteins in plants.

Proteomic Data Analysis of Plant Endoplasmic Reticulum

• Data output statistics and quality control.
• Protein identification analysis (Density map, Scatter plot, Venn diagram).
• Quantitative protein analysis (Density map, Scatter plot, Venn diagram).
• Protein GO analysis.
• Protein pathway analysis.
• Protein COG analysis.
• GO enrichment analysis of differential proteins.
• Pathway enrichment analysis of differential proteins.
• COG analysis of differential proteins.

Lifeasible offers services covering proteomic analysis in plant endoplasmic reticulum to meet your research demands. With years of experience in the biological industry, our professional platforms can help our clients solve types of difficulties. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.