Chloroplast is a type organelle conducting photosynthesis in plant and algae cells, which can convert light energy into relative stable chemical power. Photosynthesis is largely responsible for producing and maintaining the oxygen content of the Earth's atmosphere, and supplies most of the energy necessary for life on Earth.
For assisting researchers studying the regulatory mechanism of photosynthesis, Lifeasible provides gene editing services in chloroplasts.
Chloroplast is a type membrane-bound organelle in plant and algae cells. And it is considered as endosymbiotic cyanobacteria, due to the great similarity of their function, structure and close relationship in phylogeny. Most chloroplasts have their genome combined into a large circular DNA molecule, while some of them are in a linear shape. The chloroplast genome most commonly includes around 100 to 150 genes that encode many gene essential for photosynthesis, and genes regulating forming organic matter as well.
Chloroplast genetic engineering has made fruitful progresses in the development of plants, including resistance to stresses, phytoremediation of toxic metals and production of vaccine antigens, biopharmaceuticals, biofuels, biomaterials and industrial enzymes. But previous gene engineering researches were carried out with natural uptake by chloroplast genome. Popular gene editing tools, including TALE nucleases and CRISPR systems, come short of gene editing of chloroplast, because it's difficult to express two components in chloroplasts simultaneously.
Production of biopharmaceuticals
Among plant chloroplasts, tobacco chloroplast has been engineered to express genes that coding vaccine antigens and biopharmaceuticals. For example, gp120 and gp41 for HIV, GUS-E7 for human papiloma virus, CTB-VP1 for polio virus, EDIII for dengue virus and so on.
Phytoremediation
Phytoremediation is a system for cleaning up contaminated environments with plants, which is considered safe and cost-effective. Chloroplasts genetically engineered with gene coding synthesis of metal chelators have improvement in their capacity of plants for metal uptake.
Industry
Researchers have engineered chloroplast genomes to produce enzymes and biomaterials. Such as poly p-hydroxybenzoic acid polymer, poly hydroxy butyrate, endo-glucanase and so on.
Lifeasible provides gene editing services in chloroplast for assisting researchers studying mechanism of all vital activities in chloroplast, including gene knock-in and gene knock-out. Our experienced scientists dedicate to provide trustable and satisfying services for our customers.
1. Plasmids Construction
Constructing plasmid contains the reporter gene flanked by recombination recognition sequences and homology arms of the target gene, and plasmid contains another reporter gene and site-specific recombinase gene.
2. Transfection in Protoplast
The Agrobacterium-mediated transformation is the common way to introduce the plasmids into plant cells.
3. Plant Culturing
Culturing the plant from protoplast
Lifeasible offers our customers innovative, efficient, customer-tailored and high-quality gene editing services. And we promise to deliver the results and products on time and with high quality. We serve researchers all over the world with heart and soul. Please feel free to contact us.
