Cyanobacteria are one of the most ancient photosynthetic organisms on Earth. They play significant role in global biochemical cycle, including those of carbon, nitrogen and oxygen. They are important model organisms with potential applications in biotechnology.
Lifeasible is pleased to provide advanced and innovative gene editing services in cyanobacteria. We guarantee delivering the required results and products to our customers with high quality and on time.
Cyanobacteria is a burgeoning source of renewable bio-feeds, which can make a great contribution in biofuel and biosynthesis. It can directly convert sunlight in to electricity and it can also be engineered to produce algae-based fuels, such as diesel, gasoline and ethanol.
Generation of edited cyanobacteria cells is main method of gene functional study. Many of their genomes have been sequenced, but the phage research of Cyanobacteria is relatively deficient compared with other heterotrophic model organisms like E. coli, which means the suitable plasmids for cyanobacteria remains few.
Previous methods of gene editing in cyanobacteria are either sluggish or cumbersome. For example, introducing two loxP sites with two plasmids which takes long time and need to construct custom-tailored shuttle-vectors, RSF1010-based broad-host-range vector which is so large that leads to low yields and cloning difficulties and so on. And the trending CRISPR-based methods for gene editing, with Cas9 or Cas12a enzymes acting toxic for cyanobacteria, is difficult to apply.
Lifeasible provides gene editing services in cyanobacteria to handle different research purposes. Our technology is based on site-specific recombinases system for editing gene markerlessly, and we exploit the polyploidy of cyanobacteria’s genomes a natural counterselection system to carry out the markerless gene editing with only minor fitness defects.
In order to assist global customers studying the function of particular gene, we provide site-specific gene knock-out service in cyanobacteria. In four weeks, Lifeasible provides our customer genetically modified strains of interest.
Lifeasible provides gene knock-in services for assisting customers to construct different strains which can contribute in biosynthesis, organic degradation and other applications.
1. Plasmids Construction
Generally, this system comprises two plasmids. Plasmids contains the reporter gene flanked by recombination recognition sequences and homology arms of the site. Plasmids contains site-specific recombinase gene.
2. Transformation
The first plasmid should be transformed first, then identifying the cells expressing the first reporter gene. Then transforming the second plasmid to the cells expressing the first reporter gene.
3. Reporter Gene Excising
The second plasmid is transformed for excising the first reporter gene. At the end, under the absence of antibiotics, exploiting the polyploidy of the genome as a tool to excise the second reporter gene and the site-specific recombinase gene.
4. Genetically Modified Cells
The markerless genetically modified cells are generated.
Lifeasible offers our customers innovative, efficient, customer-tailored and high-quality gene editing services. And we promise to deliver the results and products on time and with high quality. We serve researchers all over the world with heart and soul. Please feel free to contact us.
