Rice Endosperm-Specific Expression System

Rice endosperm is a significant component of the grain, storing starch, protein and other nutrients required for early seed germination. Rice endosperm-specific expression system is a system that expresses explicitly exogenous or endogenous genes in rice endosperm. In the rice endosperm-specific expression system, the expression of genes in the endosperm is controlled by specific promoters. This system utilizes the rice endosperm as a " production plant " to efficiently express various types of proteins, including endosperm-specific expression, targeted storage of proteins, extraction of recombinant proteins, and purification technologies.

Lifeasible has multi-year experience in protein expression research and services. We have established a high-yield eukaryotic expression and purification system for recombinant proteins from plant seeds, and we can provide recombinant protein and peptide expression services using the rice endosperm-specific expression system.

Features of Rice Endosperm-Specific Expression System

• Low cost and high yield. Recombinant protein expression in plant cells has the advantage of lower cost than animal or microbial cell expression systems and higher yield than other plant expression systems.
• High safety. Rice, as a food source, has a high degree of safety as its endosperm-expressed proteins have a very low likelihood of being subjected to protease degradation. In addition, rice is self-pollinating, maintaining genetic purity with a traceable chain of custody from the target gene to the final biological product.
• Easy to store and transport. Plant-derived recombinant proteins are easier to store and transport than biologics that require cold chain transportation.
• High purity. The rice endosperm protein expression system contains no animal, human, or microbial by-products, making it ideal for therapeutic use or sensitive and precise biotechnology applications.

Our Service for Rice Endosperm-Specific Expression System

Generation of genetic modified crops

This process prepares the genetic basis for the expression of recombinant proteins in rice, which begins with codon optimization and gene synthesis to ensure that the gene sequence is compatible with the rice host system for optimal expression. The selection of endosperm-specific promoters ensures that recombinant proteins are specifically expressed in the rice endosperm to optimize yield. Develop suitable vector constructs to facilitate the introduction of recombinant genes into the rice genome. The transformation process is often carried out using Agrobacterium-mediated rice transformation, and the transformed rice needs to be molecularly characterized to confirm the successful integration of the recombinant gene.

Laboratory scale production

At the laboratory scale, we focus on confirming the stability and functionality of transgenic lines. Pure fit rice screening helps to ensure that modified genes are inherited stably over several generations, thus providing consistent protein expression. The biological activity of recombinant proteins needs to be rigorously tested to ensure that they perform as expected in downstream applications. At this stage, we produce microgram-scale recombinant proteins suitable for initial testing and further research.

Pilot scale production

In the pilot scale stage, the production of recombinant proteins is scaled up while maintaining high purity levels. This stage also includes a biosafety analysis of the genetically modified organism (GMO) to ensure that the genetically modified rice plant meets all the necessary environmental and agricultural safety regulatory standards prior to large-scale production.

Large-scale production

This stage involves large-scale production of recombinant proteins for industrial-scale applications. This stage involves optimizing the production process to generate sufficient quantities of high-quality recombinant proteins for commercial or research applications, ensuring consistent quality and performance.

Fig.2 Our service process. (Lifeasible)

Highlights of Our Rice Endosperm-Specific Expression System

• Simplified process. We continuously optimize our technology system, which enables us to simplify the purification process of recombinant proteins and reduce costs.
• Scale-up production capacity. We are not limited by the volume of traditional fermentation, which makes it easy to realize large-scale production, and the storage conditions of raw materials are simple for long-term preservation.
• High safety. This technical service of ours avoids the risk of animal-derived pathogens and improves product safety.
• Technology leadership. We have realized a practical breakthrough in the expression volume of plant bioreactors, which is at the international leading level.

Lifeasible is dedicated to plant research and provides comprehensive plant-based service support. We utilize the natural life cycle and growth of rice as a backdrop to create high-purity recombinant proteins and can customize individual solutions to meet your specific requirements. If you are interested, please feel free to contact us.