RNAi for Breeding Nematode Resistant Varieties

RNA interference (RNAi) has emerged as an invaluable gene-silencing tool for functional analysis in various organisms. Many studies have now described its application to plant parasitic nematodes. Genes expressed in a range of cell types are silenced when nematodes take up double stranded RNA (dsRNA) or short interfering RNAs (siRNAs) that elicit a systemic RNAi response. RNAi has become an established experimental tool for plant parasitic nematodes and also offers the prospect of being developed into a novel control strategy when delivered from transgenic plants.

Lifeasible provides RNAi for targeting plant nematodes with decades of experience. ​Our experts will answer your questions comprehensively and carefully and help you solve the problems in your studies. We guarantee satisfied and reliable results for our customers all over the world.

Breeding Nematode Resistant Model Plants with RNAi Technology

• Expression of a functional gene for root-knot nematode parasitism on the model plant of Arabidopsis thaliana can reduce the number of root knots on Arabidopsis thaliana as well as eggs per gram of root. And it is also effective against southern root-knot nematode, peanut root-knot nematode, Java root-knot nematode, and northern root-knot nematode.

Fig.1 The schematic diagram of RNAi mechanism.

• Lifeasible provides breeding services for nematode resistant model plants with RNAi technology, including in vitro / in vivo RNAi, RNAi targeting of genes overexpressed in plants, confirmation of transformation from double-stranded RNAs (dsRNAs) to siRNAs in the transgenic plant by northern hybridization.

Breeding Nematode Resistant Tobacco with RNAi Technology

Many hosts possess the basic RNAi machinery and elicit RNAi in response to dsRNA, which may benefit from these features of the host's RNAi machinery and be more effective. Moreover, this approach may directly serve as a tool to control parasite infection. We provide breeding services for nematode resistant tobacco with RNAi technology, including genes encoding splicing factors and components of chromatin remodeling complexes.

Breeding Nematode Resistant Soybean with RNAi Technology

• Transgenic soybeans are generated following transformation with an RNAi expression vector containing inverted repeats of a cDNA clone of the gene from plant nematodes. An additional bioassay evaluating progeny nematodes for maintenance of reproductive suppression indicates that progeny is also impaired in their ability to reproduce successfully.
• RNAi may have important implications for the control of other plant nematodes. We provide breeding services of nematode resistant soybean with RNAi technology, including plant tissue culture and transformation, southern blot analysis, northern blot analysis, and tests for nematode infection, etc.

Breeding Nematode Resistant Banana with RNAi Technology

Banana is a necessary staple food crop feeding but is subject to severe productivity constraints due to a range of pests and diseases. The development of improved varieties of banana, is fundamental to tackle these challenges. We provide breeding services for nematode resistant bananas with RNAi technology, applying it for the enhancement of nematode resistance by suppression of important effector proteins.

Lifeasible offers RNAi for breeding nematode resistant varieties with customized delivery strategies and precise design. Our advanced technical platforms help our clients solve the problems they may encounter in research based on decades of experience. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.