Saussurea involucrata is a perennial herb that grows mostly on the snowy plateau and is native to China and distributed in Russia and Kazakhstan. The whole herb of Saussurea involucrata can be used as medicine and has good medicinal value. In recent years, Saussurea involucrata has become an endangered plant due to its drastic decrease in population as a result of indiscriminate mining. There are many limitations in the use of conventional breeding techniques, so using plant genetic transformation technology can solve the limitations of conventional breeding and greatly promote the research and application of Saussurea involucrata.
Lifeasible provides professional plant genetic transformation services. We have established an effective Saussurea involucrata genetic transformation technology platform based on the current problems in Saussurea involucrata genetic transformation after years of experimental research. The plant tissue culture and efficient genetic transformation platform we have established can be used to detoxify and genetically transform Saussurea involucrata and other traditional Chinese medicine plants. The system has a short period, which can complete the whole process within 4-6 months, and the transformation efficiency is relatively high. In addition, we can utilize different selection markers to achieve the co-introduction of multiple genes, which establishes an excellent platform for improving plant traits using biotechnology. Currently, we obtained resistant transgenic plants by genetic transformation in Saussurea involucrata.
Plant tissue culture technology can be used for detoxification and rapid propagation of various plants, and an efficient and stable genetic transformation system is an indispensable basic technology in improving plant traits and researching plant gene functions. We have established the fast propagation of Saussurea involucrata seedlings, the suspension cell culture system, and the fast propagation system of the hairy root, and utilized the suspension cell culture system and the hairy root to produce its active ingredients, which can significantly shorten the production cycle of the medicinal ingredient of Saussurea involucrata and solve the limitations of the growth environment and growth rate of Saussurea involucrata. It can significantly shorten the production cycle of Saussurea involucrata medicinal ingredients, solve the limitations of the growth environment and growth rate of Saussurea involucrata, and provide a new way of thinking for the development and utilization of herbal medicine. Through continuous accumulation, we have accumulated rich experience in plant histoculture and genetic transformation and are capable of establishing efficient genetic transformation platforms for various plants.
In the genetic transformation of Saussurea involucrate, we mainly utilize the method of Agrobacterium transformation, in which the expression vector carrying the target gene is transformed into Agrobacterium, and this Agrobacterium is co-cultivated with Saussurea involucrata explants cultured through plant tissue culture technology so that the target gene is inserted into the genome of the explants, screened for resistance elements, and then Through molecular detection methods, the resistant plant containing the target gene is the transformed plant, and a large number of transformed plants can be obtained through the bud proliferation pathway, and the transformed plants can be cultivated to obtain a new variety of transgenic Saussurea involucrata.
Lifeasible is dedicated to plant genetic transformation research, and we have advanced molecular biology laboratories, equipment, and a team of experienced experts. In addition, we have close collaborations with several universities and companies. We can customize our plant genetic transformation services to meet our client's research goals and ensure success. If you are interested in us, please feel free to contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
