Transcriptomic analysis of fungi is the sum of all RNAs transcribed from fungi in a certain state, including mRNA and non-coding RNA. Regulation at the transcriptional level is one of the most essential regulations of organisms, and fungal transcriptomic studies are the basis for studying the regulation of fungal gene expression, whose research includes structural analysis of fungal transcripts, functional annotation of fungal genes, and discovery of new fungal transcripts.
With the rapid development of next-generation high-throughput sequencing technology, we can obtain transcriptional information about fungi in almost all states. Lifeasible can use RNA sequencing technology to obtain transcriptome information of different strains of fungi, different growth and developmental stages of fungi, and different mutants of fungi, and screen differentially expressed genes to help customers deeply understand the molecular mechanisms regulating fungal growth and development.
The growth of fungi mainly refers to the increase in the size and number of trophic fungal bodies. The development of unicellular fungi is mainly through cell expansion, nucleus division, cytoplasmic synthesis, and finally, the budding or cleavage of cells into asexual reproduction. The growth of filamentous fungi takes place in an apical extension. We can obtain information on the total RNA of fungi at different growth stages by transcriptome sequencing to understand the gene expression at different growth stages.
Comparative transcriptomes are an essential part of evolutionary genomes, and species evolution is studied by RNA sequencing technology. We can provide comparative transcriptome analysis of fungi, mainly including the screening of orthologous genes, estimation of divergence time, and identification of single copy genes, etc., to help clients understand the evolutionary relationships between different fungi and the gene expression in specific tissues.
Sexual reproduction is one of the reproductive modes of fungi and is an essential driver of genetic recombination in fungi. Mating-type loci control fungal sex and play a decisive role in sexual reproduction. Gene expression at the mating type locus varies among different types of fungi. We can obtain the mating type gene expression data of different fungi by transcriptome sequencing and help our customers to analyze the relationship between mating type loci and fungal sex control, the relationship between mating type genes and pheromone signaling pathway, and the function of the mating type genes.
Fungi sense and transmit light signals through photoreceptors, and photoreceptor studies are currently dominated by the model fungus Neurospora crassa. However, in many macrofungi, light signals can regulate the fungal physiological cycle and are necessary for the growth of protoplasts and substrates. We can obtain the gene expression of fungi under light regulation by transcriptome sequencing to help our customers find new photoreceptor genes and analyze the mechanism of photoreceptor action.
Lifeasible can obtain transcriptional information of fungi in different states through RNA sequencing technology, helping customers to promote research in the field of fungal gene function. As your trusted partner, we can meet your fungal phylogenetic analysis needs and provide you with efficient, high-quality services. If you want to know the details, please contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
