Transgenic Strategy Based on Proteinase Inhibitor Coding Genes

Proteinase inhibitors (PIs) are molecules, mostly protein in nature, which inhibit the function of proteinases/proteases released by pathogens. The proteinase inhibitors involve in perturbing the enzymatic capability in microbic chemicals that are obtainable at intervals plant pests; hence, they might not digest plant tissues. Protein inhibitors are induced mostly during wounding or chemical signaling through plant molecules. Signal molecules involved in the production and relocation of proteinase inhibitors by the phloem and xylem of the plant. When an insect feed on a plant, those inhibitors involve less digestion in the insect, thus, the pest may not grow a proper level.

Lifeasible possesses enriched experience in plant science, performing various transgenic strategies based on proteinase inhibitor coding genes. Our scientists and expert technicians have been dedicated to customizing your program to meet your requirements.

Plant Serine Protease Inhibitors

• Plant serine protease inhibitors include an abundant variety of proteins and play a fundamental role in several physiological processes. They represent between 1% and 10% of the total proteins in storage organs, being able to inhibit a different kind of serine proteases.
• In general terms, plant serine protease inhibitors regulate the activity of proteases in two different situations, regulating the activity of endogenous proteases to control and avoid indiscriminate degradation when it is not convenient. Alternatively, they act to regulate the activity of exogenous proteases, as the proteases from certain plant pathogens such as insects, fungi, bacteria, and parasites prevent cellular damage.

Fig.1 Serine proteases inhibitors identified in plants or in other organisms to increase the resistance to plant nematodes.

• Lifeasible provides transgenic strategies based on plant serine protease inhibitors from sweet potatoes and other plant species. Our service lists include the generation of transgenic plants, nematode resistance tests, southern analysis, northern analysis, and RT-PCR, assay for trypsin inhibitory activity of sporamin, etc.

Plant Cysteine Protease Inhibitors

• Cysteine proteinases have been identified as the major enzymes that function in digesting nutrients in nematodes. For this reason, genes whose products inhibit proteinases have been used for genetically engineering nematode resistant plants.
• We provide transgenic strategies based on plant cysteine protease inhibitors from rice, maize, taro, and sunflower. Our service lists include the generation of transgenic plants, molecular characterization of transgenic plants, cysteine proteinase activity assay, end chitinase activity analysis, nematode assay, and others.

Lifeasible is pleased to share our cutting-edge technology and extensive expertise in plant science to facilitate our clients' research and project development. We can offer high-quality customized services by adjusting protocols to meet specific requirements. If you are interested in our services or have any questions, please feel free to contact us or make an online inquiry.