Vitis vinifera L., also known as grape, is a woody vine in the family of Vitaceae. Grapes are famous worldwide as a fruit that can be eaten raw or made into raisins. Grapes can also be used to make wine. In addition, the roots and vines of grapes have medicinal properties that can be used to stop vomiting and calm the fetus. Grapes contain up to 10-30% sugar, mainly glucose. The various fruit acids in grapes help digestion and can strengthen the spleen and stomach. Moreover, grapes are rich in minerals, vitamin B1, vitamin B2, vitamin B6, vitamin C, and vitamin P, and essential amino acids, which are beneficial for neurasthenia and excessive fatigue. Researchers have come to realize that transgenic breeding can greatly compensate for the disadvantages of traditional breeding, such as long breeding cycles and uncertainty. The introduction of genetic transformation tools into the breeding process of grapes can produce new varieties of grapes with high carbohydrates, high yield, herbicide resistance, insect resistance, and drought tolerance.
Lifeasible has a professional grape genetic transformation platform based on professional technical service support and mainstream molecular biology experimental equipment. Moreover, our experts have had many years of research experience in plant genetic transformation, which can ensure the professionalism, stability, and efficiency of the customized transgenic platform service. We use the mainstream Agrobacterium-mediated transformation method to achieve targeted transformation by introducing target genes into the grape genome through Agrobacterium infestation. The successful transformation of grapevine seedlings needs to be identified by PCR positivity, and customers will only receive positive seedlings that meet the requirements in the end. In addition, for vector customization, we offer a variety of vector construction methods, as shown below for your selection.
Customers can provide constructed grape transformation vectors directly, and Lifeasible also offers vector construction services.
| Concentration | Volume | Other requirements |
| 80-100 ng/μL | ≥10 μL | no degradation & no contamination |
| Bacterial broth | Bacterial plate |
| Bacterial broth up to 1-year-old, preserved in glycerol | Bacterial plate activated within one week |
*For special varieties and customized services, the experiment time and the number of seeds needed may be increased. For this, please contact our staff for more information.
*Plasmids, E. coli, and Agrobacterium require cryopreservation and mailing under dry ice to avoid degradation, inactivation, and impact on experimental results.
*The strains, vectors, and receptor materials used in the experiment can be saved for free for half a year for customers, and you can pick them up at any time if you need them.
Our grape transgenesis service is staffed with dedicated experts who will provide practical advice, project design, and professional operation throughout the experiment. Our experts will keep you regularly informed of the progress of your experiment to ensure that you can follow up at any time. The entire experimental process is standardized and scalable, with the ability to customize a personalized solution to meet your grapevine trait-targeted transformation needs. All you need to do is to confirm the final experimental protocol in an in-depth discussion with our experts and submit the grape samples as required.
Lifeasible's experts have comprehensive knowledge and years of experience in solving technical problems and challenges in Vitis vinifera L. transformation. We can provide customized solutions to help you study a wide range of grape varieties. Our services guarantee the success of your project. For more information or any inquiry needs, please feel free to contact us.
Lifeasible has established a one-stop service platform for plants. In addition to obtaining customized solutions for plant genetic engineering, customers can also conduct follow-up analysis and research on plants through our analysis platform. The analytical services we provide include but are not limited to the following:
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