Wheat Germ Extract Cell-Free Expression

Wheat germ extract cell-free expression is a cell-free protein expression technology that utilizes all the components required for protein synthesis (e.g., tRNA, amino acids, enzymes, cofactors, ionic environments, etc.) in wheat germ extract for in vitro protein expression. Lifeasible relies on a state-of-the-art technology platform and an experienced team of technical experts to further optimize cell-free protein expression systems, particularly wheat germ cell-free expression systems, to produce proteins at high yields, reduce costs and scale up, including streamlining the process of extract preparation, developing novel energy regeneration systems for protein synthesis, stabilizing the substrate supply, and facilitating protein folding.

Features of Wheat Germ Extract Cell-Free Expression

• A cell-free protein expression system based on wheat germ extract significantly reduces the time required to produce proteins without cell culture and purification steps. This approach saves considerable time and increases the yield of proteins that are not only functional but also soluble.
• The wheat germ extract cell-free system is particularly favorable for the expression of toxic proteins to host cells, such as kinases or membrane-bound proteins. Since the system operates outside living cells, there is no risk of cell death or growth slowdown due to the accumulation of toxic proteins.
• The ability of the wheat germ extract-based system to integrate modified tRNAs into the translation process allows researchers to make specific modifications to proteins during translation, such as integrating non-standard amino acids or adding post-translational modifications.
• The system's flexibility also extends to the integration of unnatural amino acids (UAA) at precise locations within the protein sequence. This capability allows for introducing new chemical groups into proteins, thus facilitating the study of protein folding, interactions, and enzymatic activity.
• Systems based on wheat germ extract can be scaled up for high-throughput screening, allowing for the parallel production of a large number of different proteins at the same time.

Our Service for Wheat Germ Extract Cell-Free Expression

TNT-coupled wheat germ extraction system

We use the TNT-coupled wheat germ extraction system to improve the translation yield and throughput by integrating transcription reactions directly into the translation reaction mixture through a single test tube reaction mode.

Customized amino acid mixtures

We offer three amino acid mixtures lacking specific amino acids (methionine, cysteine, or leucine) as master mixes for reaction components to meet different protein expression needs.

Transcription factor and enzyme supply

Our systems contain tRNAs, ribosomes, initiation, elongation, and termination factors and are equipped with T7, T3, or SP6 RNA polymerases to support in vitro mRNA synthesis.

In vitro mRNA synthesis

Customers can provide plasmid DNA or PCR products, and we will synthesize mRNA in vitro using RNA polymerase, followed by incubation and detection.

Protein translation and purification

We utilize transcription factors and various enzymes required for protein synthesis in wheat germ extract, as well as supplemental amino acids, energy substances, and tRNA, to translate mRNA into proteins and provide services to purify the target proteins.

mRNA recirculation for protein synthesis

The mRNA released after translation can be recycled to synthesize proteins using a cell-free system, which can be repeated several times until the mRNA is inactivated.

Fig.2 Our service process. (Lifeasible)

Highlights of Our Wheat Germ Extract Cell-Free Expression

• Quality control. We provide strict quality control processes to ensure that the protein expression and purification process meets high standards, including SDS-PAGE, Western blot, and fluorescence signal analysis.
• Customer support. We provide professional technical support and customer service to help customers solve problems they may encounter during protein expression and ensure the smooth progress of their projects.
• Customized service. According to customers' specific needs, we can provide customized cell-free protein expression services, including specific labeling, modification, or protein expression under special conditions.

Lifeasible has accumulated rich service experience in microalgae expression system service, and our professional technical team can provide high-quality microalgae expression systems and many related special services. If you are interested, please feel free to contact us.