Y2H Library Construction and Screening Services

The yeast two-hybrid (Y2H) system allows the study of protein-protein interactions in living cells, and weak transient interactions between proteins can be sensitively detected by the expression products of reporter genes.. It is a highly sensitive technique that can be used to study interactions between proteins encoded by mammalian and plant genomes.

Figure 1. The classical yeast two-hybrid system. (Brückner, A. et al., 2009)

To meet the needs of our customers, Lifeasible offers Y2H library construction and screening services. We have many years of experience in library construction, and we have a variety of optimized total RNA extraction protocols that can effectively guarantee the purity and integrity of total RNA and ensure the diversity of samples. Our efficient library construction technology can guarantee a positive library rate of 98% or more. Our targeted vector modification enables a one-step LR reaction from nuclear to membrane protein libraries, allowing you to obtain Y2H libraries easily.

Y2H library construction services

• The general analysis process of Y2H library screening

Sample requirements

• Cell sample: cell number > 1×10⁷
• Animal samples: > 1 g
• Plant samples: > 2 g
• Total RNA: > 200 µg
• Screening service: customers provide decoy genes or constructed decoy plasmids

Finally, a Y2H library glycerol bacteria and Y2H library plasmids will be delivered. We will also deliver a detailed project report, including a library construction report, library construction pictures, a complete experimental report of the screening process, and sequencing and comparison results of the identified positive clones.

Advantages of our technology

• Experienced. We have rich experience in sample processing and library building and have accumulated 500+ high-quality yeast libraries of different species.
• Abundant types. We can build various libraries, including nuclear, membrane, and expression libraries, with only one sample.
• Choice of methods. You can build libraries using the Gateway method with specific recombination technology or use the Smart way to obtain target libraries in one step.
• Reliable quality. We provide a complete set of quality control results from total RNA to mRNA to double-stranded synthesis and purification to formal library quality control.

Please contact our staff to learn more about how we can serve you and for the opportunity to receive your customized solution.