Protocols

Agrobacterium tumefaciens-mediated Tobacco Leaf Disk Transformation

Principle Transformation of plant cells by Agrobacterium involves a series of complex reactions, including the attachment of Agrobacterium to plant cells, the release of signal molecules from plant ce

Construction of Tomato PIP Subcellular Localization Vector

Expression of some eukaryotic proteins in prokaryotic host cells is efficient and cost-effective. However, many eukaryotic proteins synthesized in bacteria have low or inactive protein activity due to

Construction of Prokaryotic Expression Vector of Cucumber LOX2 Gene

The method of inserting the cloned foreign gene into a suitable vector and then introducing it into E. coli to express a large number of proteins is generally called prokaryotic expression. Prokaryoti

Construction of Soybean NAC Cloning Vector

Cloning vectors are used to clone and amplify target gene fragments in host cells. This type of vector contains a replication signal sequence, which can replicate in host cells to obtain high-copy DNA

Chromosome Walking Technology in Rosa chinensis

Since the Arabidopsis genome has been sequenced, when studying the promoter of a certain gene, primers can be designed based on the known gene sequence and the promoter sequence of the gene can be dir

5'RACE Cloning of The Tobacco Gene SGT1

With the continuous development of biotechnology, many methods and means of cloning new genes have been developed, such as map-based cloning, transposon tagging, differential display of mRNA, genome s

3'RACE Cloning of The Tobacco Gene SGT1

With the continuous development of biotechnology, many methods and means of cloning new genes have been developed, such as map-based cloning, transposon tagging, differential display of mRNA, genome s

Polymerase Chain Reaction

Polymerase chain reaction (PCR) is a widely used molecular biology technology used to amplify specific DNA fragments in vitro. It can be regarded as special DNA replication outside organisms. Using PC

Extraction of Soluble Protein from Arabidopsis thaliana Leaves

Soluble proteins refer to proteins that can be dissolved in water or other solvents in the form of small molecules. Most of the soluble proteins in plants are enzymes involved in various metabolisms a

Extraction of Total Protein from Tomato Fruit

Protein is the executor of physiological functions and the direct embodiment of life phenomena. The study of protein structure and function will directly elucidate the change mechanism of life under p